Cytosine hydrate
WebApr 1, 1990 · Cytosine hydrate excision was investigated by assaying photoproduct release from cytosine-radiolabeled, irradiated poly (dG-dC):poly (dG-dC). Conformational shifts between B-DNA and Z-DNA were affected by heating the polymer in either nickel chloride or cobaltous chloride, and were determined by circular dichroism. WebHydrates of cytosine, deoxycytidine, CMP, or dCMP are unstable, readily reverting to the parent form by rehydration (45). However, their half-life is dramatically increased in DNA, …
Cytosine hydrate
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WebCytosine is, however, obtained in the chemical or enzymatic degradation of nucleic acids. Identification and determination is usually based on the UV absorption spectrum. Cytosine is deaminated to uracil by cytosine deaminase. That reaction is the basis for the specific determination of cytosine. WebApr 1, 2005 · Attempts to measure the UV-induced formation of 6-hydroxy-5,6-dihydrocytosine, also called “cytosine photohydrate”, within cellular DNA have been hampered by the instability of the photoproduct that can easily deaminate and/or loses a molecule of water giving rise to uracil.
Webirradiation (Radman, 1976), removes cytosine hydrate from DNA by a DNA glycosylase activity (Boorstein et al., 1989) and cleaves the phosphodiester backbone at an apurinic/apyrimidinic (AP) site via a 3-elimination reaction (Bailly and Verly, 1987; Kim and Linn, 1988; Mazumder et al., 1991). Endonuclease III has a broad WebDefine cytosine. cytosine synonyms, cytosine pronunciation, cytosine translation, English dictionary definition of cytosine. n. Abbr. C A pyrimidine base, C4H5N3O, that is the …
WebJun 13, 2024 · At 25 °C, the critical water activity for the cytosine hydrate/anhydrate system is ~0.43 and for 5-flucytosine ~0.41. In 5-flucytosine the water molecules are … WebThe nicking activity of hNth1 was stimulated more than 2-fold. The presence of near equimolar amounts of YB-1 significantly increased hNth1-catalyzed release of cytosine hydrate from UV-treated poly(dG-[3 H]dC)·poly(dG-[3 H]dC) and the release of Tg from osmium tetroxide-treated poly(dA-[3 H]dT)·poly(dA-[3 H]dT). YB-1 alone had no activity ...
WebThe monomeric damage, such as 8-oxoG or cytosine hydrate, which could be turned to AP sites by the action of DNA N-glycosylase, is generated at very low level ( Cadet et al., 2005; Friedberg et...
Webcytosine, a nitrogenous base derived from pyrimidine that occurs in nucleic acids, the heredity-controlling components of all living cells, and in some … grand forks buick dealershipWebNov 1, 1990 · These include cytosine hydrate (5,6-dihydro-6-hydroxycytosine), released as a free base by E. coli endonuclease III. The enzymic excision of cytosine hydrate by this … chinese collar t shirt full sleeveWebNov 1, 1990 · Europe PMC is an archive of life sciences journal literature. chinese collar wedding suitWebJun 1, 2016 · A novel microwave irradiation process has been developed for the synthesis of hybrid homogenised nanosized cerium oxide and cerium oxycarbonate hydrate material. Aqueous cerium(IV) sulfate and cytosine were reacted in the presence of microwave irradiation for 10–15 min at a temperature of 40–45 °C. chinese collar with 80s fashion menWebcytosine: [noun] a pyrimidine base C4H5N3O that codes genetic information in the polynucleotide chain of DNA or RNA — compare adenine, guanine, thymine, uracil. chinese collar suits for weddingWebAug 8, 1970 · Cytosine and cytidine hydrates usually showed about 3% rever- sal during titration, and CMP hydrates reversed as much as 25 O//o. The calculations of hydrate pK's were corrected for the amount of parent present during the titration. For cytosine and cytidine hydrates, an average of the initial and final amounts was used. grand forks bus depotWebMay 1, 1990 · Cytosine hydrates are most likely to occur in alternating purine:pyrimidine sequences. Such segments can adopt the Z-conformation as a result of reactions with … grand forks bx hours