Ehrlichia p28-specific antigens
WebImmunoreactive proteins of Ehrlichia canis and Ehrlichia chaffeensis that have been characterized include a family of 28-kDa major outer membrane proteins (p28) and two large antigenically divergent surface glycoprotein orthologs. WebPCR was used to amplify a 537-bp region of an Ehrlichia ewingii gene encoding a homologue of the 28-kDa major antigenic protein (P28) of Ehrlichia chaffeensis. The E. ewingii p28 gene homologue was amplified from DNA extracted from whole blood obtained from four humans and one canine with confirmed …
Ehrlichia p28-specific antigens
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WebPartial protection induced by immunization with P28-9, P28-12, and P28-19 against Ehrlichia was associated with the generation of Ehrlichia-specific cell-mediated and humoral immune responses. AB - The obligately intracellular bacterium Ehrlichia chaffeensis that resides in mononuclear phagocytes is the etiologic agent of human … WebDec 5, 2013 · Several antigens of Ehrlichia spp. have been identified based on their reactivity with immune sera from infected hosts that include the major outer membrane proteins (OMP-1/P28) encoded by a multi-gene family, ferric ion-binding protein (Fbp), disulfide bond formation (Dsb) protein, ankyrin repeat proteins, and tandem repeat …
WebFeb 1, 2024 · Ehrlichia canis is a common tick-borne intracellular pathogen causing canine monocytic ehrlichiosis (CME) in dogs worldwide. The aims of this study were to investigate the genetic diversity and antigenicity of E. canis based on the p28 and trp36 genes in dogs in Thailand. The E. canis p28 and trp36 genes were amplified by the polymerase chain … WebJan 12, 2024 · Ehrlichia detection by p28 -PCR screening from ticks. The DNA samples from all the ticks were individually screened by conventional PCR targeting p28 …
WebNov 17, 2011 · The Ehrlichia Hsp60 and P28-19 peptides are likely to induce CD4+ T cell responses as we detected antigen-specific IFN-γ-producing memory CD4+ T cells in … WebPrimers were selected from the E. ewingii p28 sequence and used to develop a species-specific PCR diagnostic assay. The p28 PCR assay amplified the expected 215-bp …
WebOct 10, 2024 · Ehrlichia bacteria are obligate intracellular members of the family Anaplasmataceae, and several species are capable of causing infection, with or without illness, in dogs. The first species described was …
WebNational Center for Biotechnology Information how to end a paragraph in an essay exampleWebThe spleens of mice vaccinated with E. muris P28-9, P28-12, P28-19, or a mixture of these three P28 proteins (P28s) using a DNA prime-protein boost regimen and challenged with … led ph8WebMar 29, 2024 · The reference standard serologic test for diagnosis of ehrlichiosis is the indirect immunofluorescence antibody (IFA) assay for immunoglobulin G (IgG). IgG IFA assays should be performed on paired acute and convalescent serum samples collected … Links with this icon indicate that you are leaving the CDC website.. The Centers … led pe sinaWebImmunoreactive proteins of Ehrlichia canis and Ehrlichia chaffeensis that have been characterized include a family of 28-kDa major outer membrane proteins (p28) and two … led ph 4.5 mmWebNov 15, 2016 · Peptides from outer membrane proteins of P30 of E. canis, OMP-1X of A. platys, and P28-19/P28-14 of E. chaffeensis were coupled to magnetic beads. The multiplex peptide assay detected antibodies in dogs experimentally infected with E. canis and E. chaffeensis, but not in an A. platys experimentally infected dog. led ph10WebEhrlichiae are obligately intracellular bacteria that reside and replicate in phagocytes by circumventing host cell defenses and modulating cellular processes, including host cell gene... led perth bibra lakeWebResult LOINC Value. Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are … led philips 32 hd smart tv 32phd6825